Generator

Part:BBa_K1758122:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-27)


RraA with N-terminal His-TEV-tag under T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The purification sequence is based on the corresponding sequence in [http://tools.thermofisher.com/content/sfs/vectors/pproexhtc_mcs.pdf pProExHTb]


Source

This part was obtained from E. coli K12 genomic DNA and cloned to pSB1C3 with Gibson assembly.

References